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賽默飛氣質(zhì)聯(lián)用儀常見的接口技術(shù)有哪些？
發(fā)布時間： 2022-07-26 點擊次數(shù)： 694次賽默飛氣質(zhì)聯(lián)用儀是指將氣相色譜儀和質(zhì)譜儀聯(lián)合起來使用的儀器。質(zhì)譜法可以進行有效的定性分析，但對復雜有機化合物的分析就顯得無能為力；而色譜法對有機化合物是一種有效的分離分析方法，特別適合于進行有機化合物的定量分析，但定性分析則比較困難。因此，這兩者的有效結(jié)合必將為化學家及生物化學家提供一個進行復雜有機化合物高效的定性、定量分析工具。像這種將兩種或兩種以上方法結(jié)合起來的技術(shù)稱之為聯(lián)用技術(shù)。目前本產(chǎn)品被廣泛應(yīng)用于復雜組分的分離與鑒定，其具有GC的高分辨率和質(zhì)譜的高靈敏度，是生物樣品中藥物與代謝物定性定量的有效工具。質(zhì)譜儀的基本部件有：三部分組成，它們被安放在真空總管道內(nèi)。接口：由GC出來的樣品通過接口進入到質(zhì)譜儀，接口是氣質(zhì)聯(lián)用系統(tǒng)的關(guān)鍵。賽默飛氣質(zhì)聯(lián)用儀常見接口技術(shù)：1、分子分離器連接：擴散型——擴散速率與物質(zhì)分子量的平方成反比，與其分壓成正比。當色譜流出物經(jīng)過分離器時，小分子的載氣易從微孔中擴散出去，被真空泵抽除，而被測物分子量大，不易擴散則得到濃縮。2、直接連接法：在色譜柱和離子源之間用長約50cm，內(nèi)徑0.5mm的不銹鋼毛細管連接，色譜流出物經(jīng)過毛細管全部進入離子源，這種接口技術(shù)樣品利用率高。3、開口分流連接：該接口是放空一部分色譜流出物，讓另一部分進入質(zhì)譜儀，通過不斷流入清洗氦氣，將多余流出物帶走。此法樣品利用率低。
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